| Symposium Schedule |
|
|
|
Click here to download the schedule Monday, June 23 (Lecture Series Day 1; Grey Rock Room in the Lory Student Center): Morning: Arrival in Fort Collins 2:00 p.m. Welcome and introduction 2:15 p.m. Fundamentals: Nucleic Acids, DNA Replication, Transcription and Translation and their Application to Assay Development (Kendra Nightingale) 3:00 p.m. Concepts in Microbial Taxonomy and Diversity and Implications for Molecular Detection (Martin Wiedmann) 3:30 p.m. Coffee Break and Vendor Exhibits 3:45 p.m. PCR: Fundamentals and Variations (PCR; Nested PCR; Multiplex PCR; Real-Time PCR; Reverse Transcriptase PCR; Controls and GLPs); Including: Hands-on Primer Design Activity (Sarita Raengpradub) 5:30 p.m. Opening Reception (from 5:30 to 6:30 pm); Keynote Address Delivered by Keith Lampel, U. S. Food and Drug Administration “Molecular-based detection of foodborne pathogens (from 6:30 to 7:15 pm); and Dinner (beginning at 7:15 pm) at the Hilton Hotel (425 W. Prospect Road) Tuesday, June 24 (Lecture Series Day 2, Grey Rock Room in the Lory Student Center): 8:00 a.m. Assay Validation and Approval, Including Use and Misuse of the Sensitivity and Specificity concepts (Mark Carter) 8:45 a.m. The Steps Before PCR: Sampling and Enrichment, Concentration Procedures, Compositing, True Real Time PCR without Enrichment (Mark Carter and Sarita Raengpradub) 9:30 a.m. The Ins and Outs of Selected Commercially Available DNA-Based Detection Assays (Martin Wiedmann) 10:00 a.m. Coffee Break and Vendor Exhibits 10:30 a.m. Applied Biosystems Molecular Detection Assays (Veronica Mankinen) 10:50 a.m. BioRad Molecular Detection Assays (Wendy Lauer) 11:10 a.m. DuPont Qualicon Molecular Detection Assays (Casey Simmons) 11:30 a.m. Idaho Technology Molecular Detection Assays (Haleigh Millward) 12:00 p.m. Working Lunch/Question and Answer Session with Company Representatives and Workshop Instructors on Selection of the Molecular Detection Assay that Fits Your Needs 1:30 p.m. Case Studies on Interpretation of Results from Nucleic Acid Based Detection Methods (Including Questions such as ”What Does a Positive PCR Assay Mean?” and “How to Deal with a PCR Positive Culture Negative Result?”) / Break-out into Groups and Troubleshoot 2:30 p.m. Considerations for Application of Molecular Detection Methods (Integration of Molecular and Cultural Methods, PCR Analysis on Enrichments, PCR on IMS beads, Quality Control) 3:30 p.m. Round Table Discussion and Wrap-up Question and Answer Session 4:30 p.m. Mixer with Happy Hour and Vendor Exhibits (Grey Rock Room, Lory Student Center) 5:30 p.m. Dinner on Your Own Wednesday, June 25 (Hands-on Workshop Day 1; Room A201 Microbiology Building): 8:00 a.m. Introduction to DNA Sequence Databases, Uploading/Downloading Sequence Data, Alignment Programs (Kendra Nightingale) 8:30 a.m. Design Custom PCR Assay for Target Organism of Interest (e.g., Salmonella, E. coli O157:H7, L. monocytogenes, Listeria spp. Pseudomonas fluorescence, Paenibacillus spp., Alicyclobacillus) and Order Primers. 10:30 a.m. Prepare Conventional Lysates for PCR Amplification of sigB for Listeria isolates and Lysates for Bax® L. monocytogenes SYBR Green Detection PCR. 12:30 p.m. Lunch on your own 1:30 p.m. Set-up sigB PCR Reactions and Begin Thermal Cycling 3:00 p.m. PCR Controls, Internal Positive Controls, Live Positive Controls, etc. 4:00 p.m. Design Multiplex PCR 5:30 p.m. Dinner on your own Thursday, June 26 (Hands-on Workshop Day 2, Room A201 Microbiology Building): 8:00 a.m. Run Gel of sigB PCR Products; Set-up Bax® L. monocytogenes SYBR Green Detection PCR; Purify sigB PCR Products, Quantify DNA, and Submit for DNA Sequencing 11:00 a.m. Set-up Custom PCR Reactions and Begin Thermal Cycling 12:00 p.m. Lunch on Your Own 1:00 p.m. Run Gel from Custom PCR 2:00 p.m. Lecture and Discussion “DNA Sequencing” (Kendra Nightingale) 3:00 p.m. Troubleshooting Custom PCR Results 4:00 p.m. Optimization of Custom PCR or Purification of Custom PCR Products and Send for Sequencing (Optional) 6:00 p.m. Workshop Closing Dinner at The Moothouse (2626 College at Drake); Mixer from 6:00 pm to 6:30 pm Followed by Dinner at 6:30 pm Friday, June 27 (Hands-on Workshop Day 3, Room A201 Microbiology Building): 8:00 a.m. Taqman PCR to Detect E. coli O157:H7 (ABI, Master Mix Kit); Run Gels from Optimization of Custom PCR. 10:00 a.m. 16S-Based Detection Strategies (Kendra Nightingale and Martin Wiedmann) 11:00 a.m. Lecture and Discussion “mRNA Detection” and hands-on activity to design TaqMan primers and probes (Sarita Raengpradub) 12:00 p.m. Lunch on Your Own 1:00 p.m. Assemble and Proofread Sequence Data and perform BLAST Searches 3:00 p.m. Optional Wrap-up Discussion Session and Individual Discussion with Instructors 5:00 p.m. Informal Happy Hour, Location TBA |
|
| Last Updated ( Wednesday, 18 June 2008 ) |
