| 2009 Symposium Schedule |
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Lecture series and symposium schedule:
All times are Mountain Standard Time Click here to download the schedule 
Monday, August 3rd (Lecture Series Day 1; Grey Rock Room in the Lory Student Center): Morning: Arrival in Fort Collins 2:00 p.m. Welcome and introduction 2:15 p.m. Fundamentals of Bacterial Genetics and Concepts in Microbial Evolution, Taxonomy and Diversity; Implications for Molecular Subtyping 3:00 p.m. Fundamentals of Subtyping: from Serotyping and other Phenotypic Methods to Nucleic Acid-based Subtyping Methods 3:45 p.m. Coffee Break and Vendor Exhibits 4:00 p.m. Fundamentals of Epidemiology/Molecular Epidemiology and their applications in foodborne disease outbreak investigations 5:45 p.m. Opening Reception (from 5:45 to 6:30 pm); Keynote Address Speaker TBA (from 6:30 to 7:15 pm); and Dinner (beginning at 7:15 pm) at the Hilton Hotel (425 W. Prospect Road) Tuesday, August 4th (Lecture Series Day 2, Grey Rock Room in the Lory Student Center): 8:00 a.m. Case Studies on Application of Molecular Subtyping; Troubleshooting, Interpretation of Results, and Implications 9:00 a.m. Ins and Outs of Commercially Available Molecular Subtyping Approaches (Automated Ribotyping, Pulsed Field Gel Electrophoresis, rep-PCR, and other Band-Based Molecular Subtyping Methods) 10:00 a.m. Coffee Break and Vendor Exhibits 10:30 a.m. Overview of 16S rDNA Sequencing for Species and Strain Identification 11:00 a.m. Applied Biosystems MicroSeq system (Veronica Mankinen) 11:25 a.m. BioRad PFGE and Subtyping Systems (TBD) 11:50 a.m. DuPont Qualicon Subtyping Systems (TBD) 12:15 p.m. bioMerieux Diversi-Lab Subtyping Systems (TBD) 12:45 p.m. Working Lunch/Question and Answer Session with Company Representatives and Workshop Instructors 1:30 p.m. Mock Outbreak Investigation (Molecular Epidemiology, Case Studies on Interpretation of Subtype Data) 4:00 p.m. Round Table Discussion and Wrap-up Question and Answer Session 4:30 p.m. Mixer with Happy Hour and Vendor Exhibits (Grey Rock Room, Lory Student Center) 5:30 p.m. Depart for an evening in Estes Park, CO (optional) Wednesday, August 5th (Hands-on Workshop Day 1; Room A201 Microbiology Building): 8:00 a.m. Introduction to PFGE; make plugs for PFGE 10:30 a.m. Plug lysis 10:45 a.m. Set-up MicroSeq PCR 12:00 p.m. Lunch on your own 1:30 p.m. Wash PFGE Plugs and Analysis of Ribotype Patterns 4:00 p.m. Set-up PFGE Plug Digests 5:30 p.m. Dinner on Your Own Thursday, August 6th (Hands-on Workshop Day 2, Room A201 Microbiology Building): 8:00 a.m. Purify MicroSseq PCR Products and Send-off for Sequencing 9:30 a.m. Load and Start Running PFGE Gel 10:30 a.m. Run rep-PCR for Diversi-Lab Typing 12:00 p.m. Lunch on Your Own 1:30 p.m. Run Bioanalyzer for rep-PCR (Diversi-Lab) 2:00 p.m. Introduction to Bioinformatics and Software for Developing Molecular Subtyping Assays (Bionumerics; Diversi-Lab; Genomics Databases; RDP) 3:00 p.m. Analyze Diversi-Lab Results 3:30 p.m. How to Design a Custom Molecular Subtyping 5:00 p.m. Break for Day 6:00 p.m. Workshop Closing Dinner Location TBA Friday, August 7th (Hands-on Workshop Day 3, Room A201 Microbiology Building): 8:00 a.m. Stain PFGE Gel 8:15 a.m. Demonstration of Sequence Assembly and Proofreading, BLAST Searches, and RDP Searches 9:00 a.m. De-stain PFGE Gel 9:15 a.m. Demonstration of Sequence Assembly and Proofreading, BLAST Searches, and RDP Searches (continued) 10:00 a.m. Photograph PFGE Gel and Analyze PFGE Patterns using Bionumerics Software 11:00 a.m. Assemble and Proofread 16s Sequences 11:45 a.m. Workshop Evaluations 12:00 p.m. Lunch on Your Own 1:30 p.m. Troubleshooting Examples (PFGE, Ribotyping, Diversi-Lab; MicroSeq) 3:00 p.m. Optional Wrap-up Discussion Session and Individual Discussion with Instructors 5:00 p.m. Informal Happy Hour, Location TBA (optional) |
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| Last Updated ( Wednesday, 11 February 2009 ) |